laser induced breakdown spectroscopy; heavy meta;, spatial distribution; phytoremediation; soil; plant;

In numerous biological experiments, there is no information about the spatial distribution of elements, especially metals, which could further serve for understanding the biochemical mechanism of their distribution and transportation. Laser induced breakdown spectroscopy (LIBS) is a useful technique for determining the spatial distribution of metals in various types of samples. Given the potential of this technique to analyze solid, liquid and gaseous samples (including aerosols), which may or may not be conductive, LIBS is used for both qualitative and quantitative purposes in many areas. In some applications, the unique properties of this technique, as the ability of micro-analysis, the possibility of analyzing a sample at a distance and in situ and quasi-non-destructive nature of analysis, are used. In this study, the basic principle of technology and its application for analysis of soils and plant tissues are discussed.

Laser Induced Breakdown Spectroscopy (LIBS); Picea abie; heavy metals;

This work is focused on application of LIBS technique for the study of plant samples. The elemental mapping on cross section throughout the annual terminal twigs of Picea abies was performed using double pulse Laser Induced Breakdown Spectroscopy (DP LIBS). 2D maps were created, where distribution of Cu and Ca in the plant tissue was observed. After mineralization of twig parts originated in the vicinity of the implementation of cross section, ICP-MS analysis was used for determination of total content of investigated elements.